Research shows that LISTERINE® Antimicrobial Mouthwash kills bacteria in the mouth within 30 seconds. If you want to notice a difference, you should use it twice daily. Studies have shown that existing bacteria causing plaque and saliva are removed within 30 seconds using LISTERINE® Mouthwash, which does not give germs a chance to remain in your mouth. Swish for a full 30 seconds to make sure you get a really clean mouth.